P-22: Protective Effect of Grapes SeedHydroalcholic Extract against Fluoxetine-Induced Fertility Toxicity in Male Mice

Background: Fluoxetine, a selective serotonin reuptake inhibitor, has been prescribed for the treatment of depression and has shown also efficacy in the treatment of bulimia nervosa and obsessive-compulsive disorder. Grape seed extract is a natural extract from the seeds of graps. It contains the most beneficial groups of plant flavonoids, proanthocyanidins oligomers. These flavonoids are potent antioxidants and exert many health-promoting effects. The present investigation was directed to study the possible chemoprotective activity of orally administered grape seed extract (GSE) against Fluxetine-induced fertility toxicity in male mice. Materials and Methods: In this study, 24 mature male mice were used. The animals divided into four groups as, control, FLX (20 mg/kg), FLX (20 mg/kg) + GSE (100 mg/kg) and GSE (100 mg/kg) orally for 42 days. The testis tissue were obtained on day 42 in all groups. Leydig cell and Mononuclear leukocytes number (MNLCN) and weight of body mice and testis tissue were measured. Results: The results revealed that the male mice exposed to fluxetine had significantly (p<0.05) reduced in (LCN) and significantly (p<0.05) increased in (MNLCN). Also weight of body and testis decreased significantly (p<0.05). Conclusion: Fluxetine can cause infertility in male mice, by the destruction of testicular tissue, Grape seed extract combined with fluxetine can reduce the negative effects of fluxetine.